{"id":10116,"date":"2022-02-03T16:37:30","date_gmt":"2022-02-03T16:37:30","guid":{"rendered":"http:\/\/TheNextWeb=1379503"},"modified":"2022-02-03T16:37:30","modified_gmt":"2022-02-03T16:37:30","slug":"ai-can-now-spot-dead-cells-100-times-faster-than-people-which-could-help-cure-alzheimers","status":"publish","type":"post","link":"https:\/\/www.londonchiropracter.com\/?p=10116","title":{"rendered":"AI can now spot dead cells 100 times faster than people \u2014 which could help cure Alzheimer\u2019s"},"content":{"rendered":"\n<p>Understanding when and why a cell dies is fundamental to the study of human development, disease, and aging. For <a href=\"https:\/\/doi.org\/10.1080\/17460441.2019.1623784\" target=\"_blank\" rel=\"nofollow noopener noreferrer\">neurodegenerative diseases<\/a> such as Lou Gehrig\u2019s disease, Alzheimer\u2019s, and Parkinson\u2019s, identifying dead and dying neurons is critical to developing and testing new treatments. But identifying dead cells can be tricky and has been a constant problem throughout <a href=\"https:\/\/scholar.google.com\/citations?hl=en&amp;user=cQdBoWUAAAAJ&amp;view_op=list_works&amp;alert_preview_top_rm=2&amp;sortby=pubdate\" target=\"_blank\" rel=\"nofollow noopener noreferrer\">my career as a neuroscientist<\/a>.<\/p>\n<p>Until now, scientists have had to manually mark which cells look alive and which look dead under the microscope. Dead cells have a <a href=\"https:\/\/doi.org\/10.1038\/cdd.2009.44\" target=\"_blank\" rel=\"nofollow noopener noreferrer\">characteristic balled-up appearance<\/a> that is relatively easy to recognize once you know what to look for. My research team and I have employed a veritable army of undergraduate interns paid by the hour to scan through thousands of images and keep a tally of when each neuron in a sample appears to have died. Unfortunately, doing this by hand is a slow, expensive, and sometimes error-prone process.<\/p>\n<figure class=\"align-center zoomable\" readability=\"2\">\n<p><figure class=\"post-image post-mediaBleed aligncenter\"><a href=\"https:\/\/images.theconversation.com\/files\/443555\/original\/file-20220131-139881-ixpc7n.png?ixlib=rb-1.1.0&amp;q=45&amp;auto=format&amp;w=1000&amp;fit=clip\" target=\"_blank\" rel=\"nofollow noopener noreferrer\"><img decoding=\"async\" loading=\"lazy\" src=\"https:\/\/images.theconversation.com\/files\/443555\/original\/file-20220131-139881-ixpc7n.png?ixlib=rb-1.1.0&amp;q=45&amp;auto=format&amp;w=754&amp;fit=clip\" sizes=\"(min-width: 1466px) 754px, (max-width: 599px) 100vw, (min-width: 600px) 600px, 237px\" alt=\"Time lapse of dying neuron over 10 minutes under a microscope\" width=\"600\" height=\"95\" class=\"js-lazy\" data-srcset=\"https:\/\/images.theconversation.com\/files\/443555\/original\/file-20220131-139881-ixpc7n.png?ixlib=rb-1.1.0&amp;q=45&amp;auto=format&amp;w=600&amp;h=95&amp;fit=crop&amp;dpr=1 600w, https:\/\/images.theconversation.com\/files\/443555\/original\/file-20220131-139881-ixpc7n.png?ixlib=rb-1.1.0&amp;q=30&amp;auto=format&amp;w=600&amp;h=95&amp;fit=crop&amp;dpr=2 1200w, https:\/\/images.theconversation.com\/files\/443555\/original\/file-20220131-139881-ixpc7n.png?ixlib=rb-1.1.0&amp;q=15&amp;auto=format&amp;w=600&amp;h=95&amp;fit=crop&amp;dpr=3 1800w, https:\/\/images.theconversation.com\/files\/443555\/original\/file-20220131-139881-ixpc7n.png?ixlib=rb-1.1.0&amp;q=45&amp;auto=format&amp;w=754&amp;h=120&amp;fit=crop&amp;dpr=1 754w, https:\/\/images.theconversation.com\/files\/443555\/original\/file-20220131-139881-ixpc7n.png?ixlib=rb-1.1.0&amp;q=30&amp;auto=format&amp;w=754&amp;h=120&amp;fit=crop&amp;dpr=2 1508w, https:\/\/images.theconversation.com\/files\/443555\/original\/file-20220131-139881-ixpc7n.png?ixlib=rb-1.1.0&amp;q=15&amp;auto=format&amp;w=754&amp;h=120&amp;fit=crop&amp;dpr=3 2262w\"><noscript><img decoding=\"async\" loading=\"lazy\" src=\"https:\/\/images.theconversation.com\/files\/443555\/original\/file-20220131-139881-ixpc7n.png?ixlib=rb-1.1.0&amp;q=45&amp;auto=format&amp;w=754&amp;fit=clip\" alt=\"Time lapse of dying neuron over 10 minutes under a microscope\" width=\"600\" height=\"95\" class srcset=\"https:\/\/images.theconversation.com\/files\/443555\/original\/file-20220131-139881-ixpc7n.png?ixlib=rb-1.1.0&amp;q=45&amp;auto=format&amp;w=600&amp;h=95&amp;fit=crop&amp;dpr=1 600w, https:\/\/images.theconversation.com\/files\/443555\/original\/file-20220131-139881-ixpc7n.png?ixlib=rb-1.1.0&amp;q=30&amp;auto=format&amp;w=600&amp;h=95&amp;fit=crop&amp;dpr=2 1200w, https:\/\/images.theconversation.com\/files\/443555\/original\/file-20220131-139881-ixpc7n.png?ixlib=rb-1.1.0&amp;q=15&amp;auto=format&amp;w=600&amp;h=95&amp;fit=crop&amp;dpr=3 1800w, https:\/\/images.theconversation.com\/files\/443555\/original\/file-20220131-139881-ixpc7n.png?ixlib=rb-1.1.0&amp;q=45&amp;auto=format&amp;w=754&amp;h=120&amp;fit=crop&amp;dpr=1 754w, https:\/\/images.theconversation.com\/files\/443555\/original\/file-20220131-139881-ixpc7n.png?ixlib=rb-1.1.0&amp;q=30&amp;auto=format&amp;w=754&amp;h=120&amp;fit=crop&amp;dpr=2 1508w, https:\/\/images.theconversation.com\/files\/443555\/original\/file-20220131-139881-ixpc7n.png?ixlib=rb-1.1.0&amp;q=15&amp;auto=format&amp;w=754&amp;h=120&amp;fit=crop&amp;dpr=3 2262w\"><\/noscript><\/a><figcaption><a href=\"https:\/\/thenextweb.com\/news\/ai-can-now-spot-dead-cells-100-times-faster-than-people-which-could-help-cure-alzheimers#\" data-url=\"https:\/\/twitter.com\/intent\/tweet?url=https%3A%2F%2Feditorial.thenextweb.com%2Fneural%2F2022%2F02%2F03%2Fai-can-now-spot-dead-cells-100-times-faster-than-people-which-could-help-cure-alzheimers%2F&amp;via=thenextweb&amp;related=thenextweb&amp;text=Check out this picture on: This is a time-lapse of what a dying neuron looks like under a microscope. Image: Jeremy Linsley\" data-title=\"Share This is a time-lapse of what a dying neuron looks like under a microscope. Image: Jeremy Linsley on Twitter\" data-width=\"685\" data-height=\"500\" class=\"post-image-share popitup\" title=\"Share This is a time-lapse of what a dying neuron looks like under a microscope. Image: Jeremy Linsley on Twitter\"><i class=\"icon icon--inline icon--twitter--dark\"><\/i><\/a>This is a time-lapse of what a dying neuron looks like under a microscope. Image: Jeremy Linsley<\/figcaption><\/figure>\n<\/p>\n<\/figure>\n<p>Making matters even more difficult, scientists recently began using <a href=\"https:\/\/doi.org\/10.1038\/nature02998\" target=\"_blank\" rel=\"nofollow noopener noreferrer\">automated microscopes<\/a> to continually capture images of cells as they change over time. While automated microscopes make it easier to take photos, they also create a massive amount of images to manually sort through. It became clear to us that manual curation was neither accurate nor efficient. Furthermore, most <a href=\"https:\/\/doi.org\/10.1038\/s41467-021-25549-9\" target=\"_blank\" rel=\"nofollow noopener noreferrer\">imaging techniques<\/a> can detect only the late stages of cell death, sometimes days after a cell has already begun to decompose. This makes it difficult to distinguish between what actually contributed to the cell\u2019s death from factors just involved in its decay.<\/p>\n<p>My colleagues and I have been trying for some time to automate the curation process. Our initial attempts could not handle the wide range of cell and microscope types we use in our research, nor rival the accuracy of our interns. But a <a href=\"https:\/\/doi.org\/10.1126\/sciadv.abf8142\" target=\"_blank\" rel=\"nofollow noopener noreferrer\">new artificial intelligence technology<\/a> my research team developed can identify dead cells with both superhuman accuracy and speed. This advance could potentially turbocharge all kinds of biomedical research, especially on neurodegenerative disease.<\/p>\n<h2>AI to the rescue<\/h2>\n<p><a href=\"https:\/\/thenextweb.com\/topic\/artificial-intelligence\" target=\"_blank\" rel=\"noopener noreferrer\">Artifical intelligence<\/a> has recently taken the field of microscopy by storm. A form of AI called <a href=\"https:\/\/doi.org\/10.1038\/nature21056\" target=\"_blank\" rel=\"nofollow noopener noreferrer\">convolutional neural networks, or CNNs<\/a>, has especially been of interest because it can analyze images as accurately as humans can.<\/p>\n<pre class=\"c-mrkdwn__pre\" data-stringify-type=\"pre\"> <figure> <p> <iframe srcdoc=\"<style>*{padding:0;margin:0;overflow:hidden}html,body{background:#000;height:100%}img{position:absolute;top:0;left:0;width:100%;height:100%;object-fit:cover;transition:opacity .1s cubic-bezier(0.4,0,1,1)}a:hover img+img{opacity:1!important}<\/style><a href='https:\/\/www.youtube.com\/embed\/YRhxdVk_sIs?feature=oembed&amp;autoplay=1&amp;mute=1&amp;modestbranding=1&amp;iv_load_policy=3&amp;theme=light&amp;playsinline=1'><img src='https:\/\/img.youtube.com\/vi\/YRhxdVk_sIs\/hqdefault.jpg'><img src='https:\/\/cdn0.tnwcdn.com\/wp-content\/themes\/cyberdelia\/assets\/img\/ytplaybtn.png' style='top: 50%;left:50%;width:68px;height:48px;transform:translate3d(-50%,-50%,0)'><img src='https:\/\/cdn0.tnwcdn.com\/wp-content\/themes\/cyberdelia\/assets\/img\/ytplaybtn-hover.png' style='top: 50%;left:50%;width:68px;height:48px;opacity:0;transform:translate3d(-50%,-50%,0)'><\/a>\" height=\"240\" width=\"320\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen frameborder=\"0\">[embedded content]<\/iframe> <\/p> <\/figure> <!--resp-video-container--><\/pre>\n<p>Convolutional neural networks can be trained to recognize and discover complex patterns in images. As with human vision, giving CNNs many example images and pointing out what features to pay attention to can teach the computer to recognize patterns of interest.<\/p>\n<p>These patterns could include <a href=\"https:\/\/doi.org\/10.1016\/j.cell.2018.03.040\" target=\"_blank\" rel=\"nofollow noopener noreferrer\">biological phenomena<\/a> difficult to see by eye. For example, one research group was able to train CNNs to identify <a href=\"https:\/\/doi.org\/10.1038\/nature21056\" target=\"_blank\" rel=\"nofollow noopener noreferrer\">skin cancer<\/a> more accurately than trained dermatologists. Even more recently, my colleagues were able to train CNNs to <a href=\"https:\/\/doi.org\/10.1016\/j.cell.2018.03.040\" target=\"_blank\" rel=\"nofollow noopener noreferrer\">identify complex biological signatures<\/a> such as cell type in microscopy images.<\/p>\n<p>Building on this work, we developed a new technology called <a href=\"https:\/\/doi.org\/10.1126\/sciadv.abf8142\" target=\"_blank\" rel=\"nofollow noopener noreferrer\">biomarker-optimized CNNs, or BO-CNNs<\/a>, to identify cells that have died. First, we needed to teach the BO-CNN to distinguish between clearly dead and clearly alive cells. So we prepared a petri dish with mice neurons that were engineered to produce a nontoxic protein called a <a href=\"https:\/\/doi.org\/10.1038\/s41467-021-25549-9\" target=\"_blank\" rel=\"nofollow noopener noreferrer\">genetically encoded death indicator, or GEDI<\/a>, that colored living cells green and dead cells yellow. The BO-CNN could easily learn that green meant \u201calive\u201d and yellow meant \u201cdead.\u201d But it was also learning other features distinguishing living and dead cells that aren\u2019t so obvious to the human eye.<\/p>\n<p>After the BO-CNN learned how to identify the characteristics that distinguished the green cells from the yellow, we showed it neurons that weren\u2019t distinguished by color. The BO-CNN was able to correctly label live and dead cells significantly faster and more accurately than people trained to do the same thing. The model could even look at images of cell types it had not seen before taken from different types of microscopes and still correctly identify dead cells.<\/p>\n<p>One obvious question still remained, however \u2013 why was our model so effective at finding dead cells?<\/p>\n<p>Researchers often treat the decisions CNNs make as <a href=\"https:\/\/doi.org\/10.1038\/s42256-019-0048-x\" target=\"_blank\" rel=\"nofollow noopener noreferrer\">black boxes<\/a>, with the strategy the computer uses to solve a visual task considered less important than how well it performs. However, because there must be some patterns in the cell structure the model focuses on to make its decisions, identifying these patterns could help scientists better define what cell death looks like and understand why it occurs.<\/p>\n<p>To figure out what these patterns were, we used additional <a href=\"https:\/\/doi.org\/10.1007\/s11263-019-01228-7\" target=\"_blank\" rel=\"nofollow noopener noreferrer\">computational tools<\/a> to create visual representations of the BO-CNN\u2019s decisions. We found that our BO-CNN model detects cell death in part by focusing on changing fluorescence patterns in the nucleus of the cell. This is a feature that human curators were previously unaware of, and it may be the reason designs for previous AI models were less accurate than the BO-CNN.<\/p>\n<figure class=\"align-center zoomable\" readability=\"6\">\n<p><figure class=\"post-image post-mediaBleed aligncenter\"><a href=\"https:\/\/images.theconversation.com\/files\/443244\/original\/file-20220128-14047-1wva32o.png?ixlib=rb-1.1.0&amp;q=45&amp;auto=format&amp;w=1000&amp;fit=clip\" target=\"_blank\" rel=\"nofollow noopener noreferrer\"><img decoding=\"async\" loading=\"lazy\" src=\"https:\/\/images.theconversation.com\/files\/443244\/original\/file-20220128-14047-1wva32o.png?ixlib=rb-1.1.0&amp;q=45&amp;auto=format&amp;w=754&amp;fit=clip\" sizes=\"(min-width: 1466px) 754px, (max-width: 599px) 100vw, (min-width: 600px) 600px, 237px\" alt=\"Microscopy images showing rat neurons before and after treatment with glutamate; the neurons are colored green when alive and yellow when dead\" width=\"600\" height=\"354\" class=\"js-lazy\" data-srcset=\"https:\/\/images.theconversation.com\/files\/443244\/original\/file-20220128-14047-1wva32o.png?ixlib=rb-1.1.0&amp;q=45&amp;auto=format&amp;w=600&amp;h=354&amp;fit=crop&amp;dpr=1 600w, https:\/\/images.theconversation.com\/files\/443244\/original\/file-20220128-14047-1wva32o.png?ixlib=rb-1.1.0&amp;q=30&amp;auto=format&amp;w=600&amp;h=354&amp;fit=crop&amp;dpr=2 1200w, https:\/\/images.theconversation.com\/files\/443244\/original\/file-20220128-14047-1wva32o.png?ixlib=rb-1.1.0&amp;q=15&amp;auto=format&amp;w=600&amp;h=354&amp;fit=crop&amp;dpr=3 1800w, https:\/\/images.theconversation.com\/files\/443244\/original\/file-20220128-14047-1wva32o.png?ixlib=rb-1.1.0&amp;q=45&amp;auto=format&amp;w=754&amp;h=445&amp;fit=crop&amp;dpr=1 754w, https:\/\/images.theconversation.com\/files\/443244\/original\/file-20220128-14047-1wva32o.png?ixlib=rb-1.1.0&amp;q=30&amp;auto=format&amp;w=754&amp;h=445&amp;fit=crop&amp;dpr=2 1508w, https:\/\/images.theconversation.com\/files\/443244\/original\/file-20220128-14047-1wva32o.png?ixlib=rb-1.1.0&amp;q=15&amp;auto=format&amp;w=754&amp;h=445&amp;fit=crop&amp;dpr=3 2262w\"><noscript><img decoding=\"async\" loading=\"lazy\" src=\"https:\/\/images.theconversation.com\/files\/443244\/original\/file-20220128-14047-1wva32o.png?ixlib=rb-1.1.0&amp;q=45&amp;auto=format&amp;w=754&amp;fit=clip\" alt=\"Microscopy images showing rat neurons before and after treatment with glutamate; the neurons are colored green when alive and yellow when dead\" width=\"600\" height=\"354\" class srcset=\"https:\/\/images.theconversation.com\/files\/443244\/original\/file-20220128-14047-1wva32o.png?ixlib=rb-1.1.0&amp;q=45&amp;auto=format&amp;w=600&amp;h=354&amp;fit=crop&amp;dpr=1 600w, https:\/\/images.theconversation.com\/files\/443244\/original\/file-20220128-14047-1wva32o.png?ixlib=rb-1.1.0&amp;q=30&amp;auto=format&amp;w=600&amp;h=354&amp;fit=crop&amp;dpr=2 1200w, https:\/\/images.theconversation.com\/files\/443244\/original\/file-20220128-14047-1wva32o.png?ixlib=rb-1.1.0&amp;q=15&amp;auto=format&amp;w=600&amp;h=354&amp;fit=crop&amp;dpr=3 1800w, https:\/\/images.theconversation.com\/files\/443244\/original\/file-20220128-14047-1wva32o.png?ixlib=rb-1.1.0&amp;q=45&amp;auto=format&amp;w=754&amp;h=445&amp;fit=crop&amp;dpr=1 754w, https:\/\/images.theconversation.com\/files\/443244\/original\/file-20220128-14047-1wva32o.png?ixlib=rb-1.1.0&amp;q=30&amp;auto=format&amp;w=754&amp;h=445&amp;fit=crop&amp;dpr=2 1508w, https:\/\/images.theconversation.com\/files\/443244\/original\/file-20220128-14047-1wva32o.png?ixlib=rb-1.1.0&amp;q=15&amp;auto=format&amp;w=754&amp;h=445&amp;fit=crop&amp;dpr=3 2262w\"><\/noscript><\/a><figcaption><a href=\"https:\/\/thenextweb.com\/news\/ai-can-now-spot-dead-cells-100-times-faster-than-people-which-could-help-cure-alzheimers#\" data-url=\"https:\/\/twitter.com\/intent\/tweet?url=https%3A%2F%2Feditorial.thenextweb.com%2Fneural%2F2022%2F02%2F03%2Fai-can-now-spot-dead-cells-100-times-faster-than-people-which-could-help-cure-alzheimers%2F&amp;via=thenextweb&amp;related=thenextweb&amp;text=Check out this picture on: These images show alive neurons colored green and dead neurons colored yellow. To induce death, neurons were treated with an excess of the neurotransmitter glutamate, overstimulating them to the point of irreversible damage. Image: Jeremy Linsley\" data-title=\"Share These images show alive neurons colored green and dead neurons colored yellow. To induce death, neurons were treated with an excess of the neurotransmitter glutamate, overstimulating them to the point of irreversible damage. Image: Jeremy Linsley on Twitter\" data-width=\"685\" data-height=\"500\" class=\"post-image-share popitup\" title=\"Share These images show alive neurons colored green and dead neurons colored yellow. To induce death, neurons were treated with an excess of the neurotransmitter glutamate, overstimulating them to the point of irreversible damage. Image: Jeremy Linsley on Twitter\"><i class=\"icon icon--inline icon--twitter--dark\"><\/i><\/a>These images show alive neurons colored green and dead neurons colored yellow. To induce death, neurons were treated with an excess of the neurotransmitter glutamate, overstimulating them to the point of irreversible damage. Image: Jeremy Linsley<\/figcaption><\/figure>\n<\/p>\n<\/figure>\n<h2>Harnessing the power of AI<\/h2>\n<p>I believe our approach represents a major advance in harnessing artificial intelligence to study complex biology, and this proof of concept could be broadly applied beyond detecting cell death in microscopic imaging. <a href=\"https:\/\/github.com\/finkbeiner-lab\/GEDI-ORDER\" target=\"_blank\" rel=\"nofollow noopener noreferrer\">Our software is open-source<\/a> and available to the public.<\/p>\n<p>Live-cell microscopy is extremely rich with information that researchers have difficulty interpreting. But with the use of technologies like BO-CNNs, researchers can now use signals from cells themselves to train AI to recognize and interpret signals in other cells. By taking out human guesswork, BO-CNNs increase the reproducibility and speed of research and can help researchers discover new phenomena in images that they would otherwise not have been able to easily recognize.<\/p>\n<p>With the power of AI, my research team is currently working to extend our BO-CNN technology toward predicting the future \u2013 identifying damaged cells before they even start to die. We believe this could be a game-changer for neurodegenerative disease research, helping pinpoint new ways to prevent neuronal death and eventually lead to more effective treatments.<!-- End of code. If you don't see any code above, please get new code from the Advanced tab after you click the republish button. The page counter does not collect any personal data. More info: https:\/\/theconversation.com\/republishing-guidelines --><\/p>\n<p><em>This article by <a href=\"https:\/\/theconversation.com\/profiles\/jeremy-linsley-1301141\" target=\"_blank\" rel=\"nofollow noopener noreferrer\">Jeremy Linsley<\/a>, Scientific Program Leader at Gladstone Institutes, <a href=\"https:\/\/theconversation.com\/institutions\/university-of-california-san-francisco-689\" target=\"_blank\" rel=\"nofollow noopener noreferrer\">University of California, San Francisco<\/a> is republished from <a href=\"https:\/\/theconversation.com\" target=\"_blank\" rel=\"nofollow noopener noreferrer\">The Conversation<\/a> under a Creative Commons license. Read the <a href=\"https:\/\/theconversation.com\/new-ai-technique-identifies-dead-cells-under-the-microscope-100-times-faster-than-people-can-potentially-accelerating-research-on-neurodegenerative-diseases-like-alzheimers-174154\" target=\"_blank\" rel=\"nofollow noopener noreferrer\">original article<\/a>.<\/em><\/p>\n<p> <a href=\"https:\/\/thenextweb.com\/news\/ai-can-now-spot-dead-cells-100-times-faster-than-people-which-could-help-cure-alzheimers\">Source<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Understanding when and why a cell dies is fundamental to the study of human development, disease, and aging. For neurodegenerative diseases such as Lou Gehrig\u2019s disease, Alzheimer\u2019s, and Parkinson\u2019s, identifying dead and&#8230;<\/p>\n","protected":false},"author":1,"featured_media":10117,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/www.londonchiropracter.com\/index.php?rest_route=\/wp\/v2\/posts\/10116"}],"collection":[{"href":"https:\/\/www.londonchiropracter.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.londonchiropracter.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.londonchiropracter.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.londonchiropracter.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=10116"}],"version-history":[{"count":0,"href":"https:\/\/www.londonchiropracter.com\/index.php?rest_route=\/wp\/v2\/posts\/10116\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.londonchiropracter.com\/index.php?rest_route=\/wp\/v2\/media\/10117"}],"wp:attachment":[{"href":"https:\/\/www.londonchiropracter.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=10116"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.londonchiropracter.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=10116"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.londonchiropracter.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=10116"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}